human −670 to cdh1 promoter/luciferase reporter construct #42083  (Addgene inc)

Journal: Cancer letters

Article Title: The NF-Y splicing signature controls hybrid EMT and ECM-related pathways to promote aggressiveness of colon cancer.

doi: 10.1016/j.canlet.2023.216262

Figure Lengend Snippet: Fig. 7. Overview of the molecular activity and clinical implications of high NF–YAl or NF–YAs expression in CRC. High expression of NF–YAs and NF–YAl differently modulates the transcription of ECM- and EMT-related genes. In particular, direct transcriptional regulation of E-Cadherin by NF–YAs and NF–YAl confers diverse biological properties to CRC cells, in terms of growth and migration. NF-YAlhigh levels enhance metastatic dissemination of CRC cells, characterize aggressive tumors and predict poor overall survival of CRC patients. Created with BioRender.com.

Article Snippet: 250 ng of the Cdh1 promoter luciferase construct (proE-cad178-Luc, Addgene plasmid # 42081 [34]) were co-transfected with 100 ng of pCMV GFP and 100-200-300 ng of pSG5-NF-YA-DN G. Rigillo et al. Cancer Letters 567 (2023) 216262 vector encoding for the NF-YA dominant negative mutant or pSG5 expression vector encoding for murine NF–YAl and NF–YAs [35,36].

Techniques: Activity Assay, Expressing, Migration